DNAPac PA100、PA200 Column >>了解產品規格及報價
Thermo DNA/RNA 核酸藥物分析管柱總覽
* Measured using a 20mer oligonucleotide
** Measured using a 25mer oligonucleotide
Thermo DNAPac PA100、PA200、PA200 RS 管柱的顆粒技術與優勢
A strong anion exchange column developed to provide high-resolution analysis and purification of synthetic oligonucleotides
• High-resolution oligonucleotide separations
• Achieve n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Suitable for the analysis of phosphorothioate-based clinical samples
• Easy scale-up from 2.0mm to 22mm ID column (>100x)
The DNAPac PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized Thermo Scientific™ Dionex™ MicroBeads™. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations. DNAPac PA100 can resolve full length from n–1, n+1, and other failure sequences.
DNAPac PA200強陰離子交換管柱，解析度可到 n-1, n, n+1 oligonucleotides，可分析或是純化oligonucleotides，包含RNA (2 -5 )及(3 -5 )links、DNA修飾之應用。
A strong anion exchange column developed to provide the best resolution for analysis and purification of synthetic oligonucleotides
• Achieve n, n-1 resolution for oligonucleotides
• Resolve oligonucleotides with secondary structures
• Assay phosphorothioate purity
• Selectivity control with eluent pH, salt, and solvent
• Resolve RNA with aberrant (2’, 5’) links from normal ssRNA
• Separate individual phosphorothioate diastereoisomers
• HR/AM AXLC/MS via automated desalting
• 9-fold more stable to high pH than DNAPac PA100
The DNAPac PA200 is packed with a pellicular anion-exchange resin composed of an 8μm diameter nonporous polymeric substrate to which quaternary amine-functionalized Dionex MicroBeads are bound. The rapid mass transport characteristics of this resin result in high-resolution oligonucleotide separations. DNAPac PA200 can resolve full length from n−1, n+1, and other failure sequences not possible with other columns.
DNAPac PA200 RS強陰離子交換管柱，粒徑更小解析力更高，能分析到80-mer的oligonucleotides。管柱耐壓最大10000psi能支援到UHPLC系統。
UHPLC Solutions for nucleic acid analysis
• Provide single base resolution of oligonucleotides
• Higher efficiency to improve resolution
• Improved throughput
• Ruggedness consistent with the DNAPac PA200 column line
• Stable to 10,000 psi
DNAPac PA200 RS columns are packed with smaller, 4μm particles, for improved resolution and better performance. The smaller particles also allow shorter columns to provide significantly higher throughput. These columns are packed in Bio-Inert PEEK-lined stainless steel (SST) bodies, designed to protect from unwanted interactions of eluents and analytes with metals, while maintaining 10,000 psi stability. These columns offer exceptional resolution of oligonucleotides, including isomer separations; and are able to resolve full length oligonucleotides from n-1 and n+1 oligonucleotides and other failure sequences.
